Effects of L-Carnitine on Cardiac Apoptosis in Ischemic- Reperfused Isolated Rat Heart
نویسندگان
چکیده مقاله:
Carnitine is a vital biologic substance for transporting fatty acids into myocytes. It also facilitates fatty acids β-oxidation for energy production. In this study, effects of L-carnitine (L-Car) on apoptosis in the ischemic isolated rat heart were investigated. Male Sprague-Dawley rats were divided into four groups and anesthetized by sodium pentobarbital. The heart was removed and mounted on a Langendorff apparatus then perfused by a modified Krebs-Henseleit (K/H) solution under a constant pressure at 37 °C. In the control group, the hearts were perfused only by normal K/H solution at stabilization, 30 min. regional ischemia and 120 min. reperfusion, while in each of the test groups, the hearts were perfused during ischemia-reperfusion with 0.5, 2.5 and 5 mM of L-Car-enriched K/H solution, respectively. At the end of reperfusion, immunohistochemical detection of apoptotic cells was performed by using an in situ apoptosis detection kit. The number of TUNEL-positive cardiomyocytes was counted in five random high-power fields in each sample. In the control group, the number of apoptotic cells were 48±3 while addition of L-Car (0.5, 2.5 and 5 mM) to the solution reduced the number of apoptotic cells to 6±1, 4±1 and 3±1, respectively (p
منابع مشابه
effects of l-carnitine on cardiac apoptosis in ischemic- reperfused isolated rat heart
carnitine is a vital biologic substance for transporting fatty acids into myocytes. it also facilitates fatty acids β-oxidation for energy production. in this study, effects of l-carnitine (l-car) on apoptosis in the ischemic isolated rat heart were investigated. male sprague-dawley rats were divided into four groups and anesthetized by sodium pentobarbital. the heart was removed and mounted on...
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متن کاملeffects of acetyl-l-carnitine on cardiac arrhythmias and infarct size in ischemic-reperfused isolated rat heart
objective(s) this study aimed to examine whether acetyl-l-carnitine (alc) was able to reduce cardiac arrhythmias and infarct size in the ischemic-reperfused isolated rat heart. materials and methods the isolated hearts were mounted on a langendorff apparatus then perfused by a modified krebs-henseleit solution during 30 min regional ischemia and 120 min reperfusion (control) or by enriched kreb...
متن کاملEffects of Acetyl-L-Carnitine on Cardiac Arrhythmias and Infarct Size in Ischemic-Reperfused Isolated Rat Heart
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Comparison between the effects of L-Carnitine and Acetyl-L-Carnitine on myocardial infarction size in ischemic heart
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عنوان ژورنال
دوره 3 شماره 1
صفحات 19- 24
تاریخ انتشار 2007-01-01
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